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FlowJo Basic Tutorial - flowlab-childrens-harvard.com

FlowJo Basic Tutorial FlowJo is software for the analysis of flow cytometry data. this Tutorial will quickly introduce you to FlowJo using an example experiment, where a FITC anti-CD8 reagent is titrated to determine the most economical concentration for staining in future experiments. Table of Contents Introduction .. 1. Step 1: Load samples into the workspace .. 3. Step 2: Analyze the control sample in detail .. 7. Step 3: Copy gates and statistics to all samples .. 12. Step 4: Verify gates on all samples .. 13. Step 5: Generate a graphical report .. 17. Step 6: Generate a table of statistics .. 23. FlowJo is a product of Tree Star, Inc. USA: (800) 366-6045. International: 1 541-201-0022. FlowJo was written by Adam Treister and Mario Roederer in 1996, based on concepts developed at the Herzenberg laboratory at Stanford.

Basic Tutorial Introduction FlowJo software is used for the analysis of flow cytometry data. You can use FlowJo to analyze all of your flow cytometry data - regardless of the cytometer used to collect your data files. This tutorial will introduce you to FlowJo and to the 6 steps involved in analyzing a basic immunophenotyping experiment. 1.

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