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Fluorophore selection guide for flow cytometry - Inserm

Flow CytometryFluorophore selection guide for flow cytometryDye choices for your instrument made selection guide for flow cytometryDyes for the 405 nm and 407 nm lasers .. 2 Dyes for the 488 nm laser.. 3 Dyes for the 635 nm laser.. 5 Accuri C6 Flow Cytometer System .. 6BD FACSC alibur System .. 7BD FACScan Flow Cytometer.. 8BD FACSC anto System.. 9BD FACSC anto II System, 2-laser option .. 11BD FACSC anto II System, 3-laser option .. 13BD LSR Flow Cytometer .. 15BD LSR II Flow Cytometer .. 17BD FACSAria Cell Sorter .. 19BD FACSA rray Bioanalyzer System.. 21BD FACSV antage SE Cell Sorter.. 22 Beckman Coulter Cytomics FC 500 Flow Cytometer.

→ Energy transfer dye excited at ~488 nm with emission maximum at ~670 nm → Also known as PE-Cy®5 → Bright fluorescence → Low compensation against RPE → Significant emission in APC channel with 633 nm excitation → Measured in FL3 channel on BD FACScan™ and BD FACSCalibur™ instruments

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