Transcription of Fundamentals of UV-Visible Spectroscopy (5965-5123E)
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Fundamentals of UV-Visible Spectroscopy (5965-5123E)
: Page i Friday, December 20, 1996 4:12 PM. Contents Chapter 1 Principles and applications of UV-Visible Spectroscopy Basic 10. The electromagnetic 10. Wavelength and frequency .. 11. Origin of UV-Visible spectra .. 11. Transmittance and absorbance .. 14. Derivative 14. Obtaining derivative spectra .. 16. Applications .. 17. Signal-to-noise .. 17. Instrumental considerations .. 18. Qualitative 18. Identification spectra and structure .. 18. Confirmation of identity .. 19. 21. Other qualitative information .. 22. Protein and nucleic acid melting 22. Enzyme activity .. 23. Instrumental 24. Quantitative 24. Beer's 24. Sample 28. Multicomponent analysis .. 29. Principle of additivity .. 29. Simple simultaneous equations 29.
UV-visible spectroscopy. These provide valuable insight into the uses and limitations of this technique for chemical analysis. The primary applications of UV-visible spectroscopy are also briefly reviewed. Basic principles The electromagnetic spectrum Ultraviolet (UV) …
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