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Gen5 Sample Protocols and Experiments Guide

Gen5. Sample Protocols &. Experiments Guide 2006 BioTek Table of Contents 5. Absorbance .. 7. 260/280 Ratio Test .. 8. AlamarBlue (Absorbance) .. 9. BCA Protein Assay .. 10. BioCell Layout protocol .. 11. Bradford Protein Assay .. 12. Direct dsDNA Quantitation .. 13. Direct Oligonucleotide Quantitation .. 14. Direct RNA 15. EDTA Inhibition Assay .. 16. IC50 (Toxicity) Assay .. 17. Kinetic Analysis -Galactosidase with ONPG 18. Kinetic Endotoxin 19. Lactate NADH Assay .. 20. Lowry Protein Assay .. 21. Particulate Test A320 .. 22. Phenol 23. Fluorescence .. 25. AlamarBlue (Fluorescent).

Experiment file name: BCA Protein Assay with data.xpt The experiment file contains the absorbance determination. Transformed (blank-subtracted) data is used to plot the standard curve. Plate Configuration: The plate layout includes standards in duplicate, with concentrations of 0 to 100 µg/ml placed in wells A2 through B6.

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