Transcription of General Chapter 61 - USP–NF | USP-NF
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General Chapter 61 - USP–NF | USP-NF
USP 31 Microbiological Tests / 61 Microbiological Examination1 Preparation of Test Strains 61 MICROBIOLOGICALUse standardized stable suspensions of test strains or prepare asEXAMINATION OF NONSTERILE stated below. Seed-lot culture maintenance techniques (seed-lot sys-tems) are used so that the viable microorganisms used for inocula-PRODUCTS:MICROBIAL tion are not more than 5 passages removed from the original masterseed-lot. Grow each of the bacterial and fungal test strains sepa-ENUMERATION TESTS rately as described in Table 1. Use Buffered Sodium Chloride Peptone Solution pH orPhosphate Buffer Solution pH to make test suspensions; to sus-pend A.
Candida albicans such Sabouraud Dextrose Soybean±Casein Sabouraud Soybean±Casein Sabouraud as ATCC 10231, Agar or Sabouraud Digest Agar Dextrose Agar Digest Agar Dextrose Agar NCPF 3179, IP Dextrose Broth £ 100 cfu £ 100 cfu £ 100 cfu £ 100 cfu 48.72, or NBRC 20°±25° 30°±35° 20°±25° 30°±35° 20°±25°
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