Transcription of Genetic Engineering Methods - Oregon State University
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Genetic Engineering Methods - Oregon State University
Genetic Engineering MethodsOutline Why do it? Research examples: poplar trees Plant gene transfer concepts and Methods Getting genes ready for transfer (recombinant DNA/plasmids) Analysis of transgenic plantsWhy do it? To create new, desired trait not in accessible gene pool To breed faster, more directly Dominant, rare alleles To amplify Genetic diversity for particular genes/traits for breeding To breed with more direction, science basis To use plants as bioproductfactories for industry/medicine To promote biosafety for exotic/unsafe crop (domestication, sterility) In all cases, new traits/diversity feeds into breeding to check yield/stability of whole organism in the field: integrated health testNative gene alteration example:Glutamine synthetase(GS) over-expression stimulates growth of poplars Hyper-expression of GS to stimulate ammonium nitrogen incorporation into proteins 3-year field trial (Spain) Increased nitrogen storage in stem Tree height up 41% Many examples, abiotic stress(F.)
To amplify genetic diversity for particular genes/traits for breeding ¾To breed with more direction, science basis To use plants as bioproduct factories for industry/medicine To promote biosafety for exotic/unsafe crop (domestication, sterility) In all cases, new traits/diversity feeds into breeding to check yield/stability of whole
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