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Genomic DNA clean-up - mn-net.com
Genomic DNA. clean -up User manual NucleoSpin gDNA clean -up July 2017 / Rev. 03. gDNA clean -up Protocol-at-a-glance (Rev. 03). NucleoSpin gDNA clean Up 150 L sample + 450 L DB. 1 Adjust DNA binding Vortex 5 s conditions (For smaller sample volumes adjust to 150 L with water, for larger sample volumes increase binding buffer proportionally.). Load sample on NucleoSpin . gDNA clean -up Column 2 Bind DNA. 11,000 x g 30 s + 700 L DW. Vortex 2 s 1st wash 11,000 x g 30 s 3 Wash silica membrane + 700 L DW. Vortex 2 s 2nd wash 11,000 x g 30 s 11,000 x g 4 Dry silica membrane 1 min 50 L DE. RT. 1 min 11,000 x g 5 Elute DNA 30 s (Optional: Repeat elution with first eluate or another 50 L of fresh Buffer DE. Heating elution buffer to 70 C.)
6 MACHEREY-NAGEL – 07/2017, Rev. 03 gDNA clean-up 2.3 Removal of RNA Nucleotides and small oligonucleotides are removed by the gentle binding conditions and
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