Transcription of PCR clean-up Gel extraction - Macherey-Nagel AG
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PCR clean-up Gel extraction - Macherey-Nagel AG
PCR clean -up Gel extraction User manual NucleoSpin Gel and PCR clean -up February 2017 / Rev. 04. PCR clean -up, gel extraction Protocol-at-a-glance (Rev. 04). PCR Gel DNA clean -up Single stranded clean -up extraction (with SDS) DNA clean -up 1 PCR clean -up, DNA clean -up, or single stranded DNA clean -up: Adjust binding condition Gel extraction : Excise DNA 200 L NTI/ 200 L NTI/ 500 L NTB/ 200 L NTC/. fragment / 100 L PCR 100 mg gel 100 L sample 100 L sample solubilize gel slice 50 C. 5 10 min 11,000 x g 2 Bind DNA. 30 s 700 L NT3. 11,000 x g 30 s 3 Wash silica Recommended: membrane 2nd wash 700 L NT3.
MACHEREY-NAGEL – 02/2017, Rev. 04 5 PCR clean-up, gel extraction 1.2 Reagents, consumables, and equipment to be supplied by user Reagents • 96–100 % ethanol
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