Transcription of 제한효소 / Ligation을 이용한 Cloning - TaKaRa CMS
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제한효소 / Ligation을 이용한 Cloning - TaKaRa CMS
[ ]1. Insert DNA ( DNA )(a) DNA cutting DNA , plasmid vector Cloning . Hind lll BamH I double digestion . DNA ( 1 ) Hind lll 1 BamH I 1 10 x K buffer 2 dH2O up to 20 ( ) 37 C 1 . Agarose gel . (10 ) loading buffer . DNA gel . DNA , UV . gel DNA ( gel extraction kit ). insert DNA A (b) PCR Vector ligation insert primer 5 . primer insert DNA PCR . + 5 3 base nucleotide TaKaRa Ex Taq Hot Start Version PCR.
[실험방법] 1. Insert DNA 준비 (목적 DNA 단편 조제) (a) 제한효소를 이용한 DNA의 cutting 목적 DNA 단편의 제한효소 사이트를 확인한 후, 사용할 plasmid
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