Transcription of 새로운 Mammalian Expression vector EF1αPromoter Vector …
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새로운 Mammalian Expression vector EF1αPromoter Vector …
2 continued . Mammalian Expression Vector EF1 Promoter Vector . Human elongation factor-1 alpha (EF1 alpha) human (2) Lentiviral system . constitutive promoter , in vitro , in vivo . 1). , EF1 promoter . silencing Mouse ES cell line E14, D3 Clontech Lenti-X Lenviral system . 2). Takara Bio Clontech CMV promoter EF1 promoter AcGFP1. EF1 Promoter Vector plasmid . 5 FACS FL1 channel . AcGFP1 . EF1 promoter . (1) Mouse ES cell transient stable Expression cell line CMV promoter EF1 promoter . AcGFP1 ( 2). Wang et al ., 2008 CMV promoter EF1 . AcGFP1 pBApo-EF1 Neo pBApo-CMV Neo promoter silencing . (TaKaRa Code 3240) Xfect Stem Transfection Reagent (TaKaRa . Code 631320) mouse ES (E14TG2a) transient . 48 E14TG2a Flow cytometer AcGFP1 (transient Expression ).
34 · Life Science & Biotechnology 새로운 Mammalian Expression vector EF1αPromoter Vector 시리즈 Human elongation factor-1 alpha (EF1 alpha)는 human 유래의
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