Transcription of 역전사 효소 & cDNA 합성 - TaKaRa CMS
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역전사 효소 & cDNA 합성 - TaKaRa CMS
25 & cDNA RNA RT-PCR library , (Reverse Transcriptate: RTase) cDNA . MMLV RTase 1st Strand cDNA .Cloning avian myeloblastosis virus(AMV) moloney murineleukemia virus(M-MLV) 2 . mRNA AMV RTase , MMLV (PrimeScript RTase ) MMLV . cDNA RNA annealing 3 primer .. Oligo dT primer: mRNA polyA tail annealing , 3 cDNA . Random primer( 6nt~ 9nt ): mRNA cDNA . 3. Specific primer: cDNA .[ ]1st strand cDNA PrimeScript Reverse Transcriptase Tube RNA/Primer .Oligo dT primer 50 pmol ( Random primer (6 mers 50 pmol ) ( Gene specific primer 2 pmol )dNTP mixture 10 mM each 1 Total RNA 1 5 ( mRNA 1 )RNase free dH2O Up to 10 65 C 5 incubation.)
25 역전사 효소 & cDNA 합성 정제된 RNA로부터 RT-PCR 또는 library를 제작할 경우, 우선 역전사 효소(Reverse Transcriptate: RTase)를 이용해 cDNA를 합성한다.
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