Transcription of 역전사 효소 & cDNA 합성 - TaKaRa CMS
1 25 & cDNA RNA RT-PCR library , (Reverse Transcriptate: RTase) cDNA . MMLV RTase 1st Strand cDNA .Cloning avian myeloblastosis virus(AMV) moloney murineleukemia virus(M-MLV) 2 . mRNA AMV RTase , MMLV (PrimeScript RTase ) MMLV . cDNA RNA annealing 3 primer .. Oligo dT primer: mRNA polyA tail annealing , 3 cDNA . Random primer( 6nt~ 9nt ): mRNA cDNA . 3. Specific primer: cDNA .[ ]1st strand cDNA PrimeScript Reverse Transcriptase Tube RNA/Primer .Oligo dT primer 50 pmol ( Random primer (6 mers 50 pmol ) ( Gene specific primer 2 pmol )dNTP mixture 10 mM each 1 Total RNA 1 5 ( mRNA 1 )RNase free dH2O Up to 10 65 C 5 incubation.)
2 2 total 20 . : RNA/Primer Mixture 10 5X PrimeScript Buffer 4 RNase Inhibitor 20 unitsPrimeScript Reverse Transcriptase 100~200 units RNase free dH2O up to 20 votex ..Option (30 C 10 min) *342 C (~ 50 C) *4 30 ~ 60 min 70 C 15 incubation . 1st strand cDNA PCR 2nd strand cDNA . ! *11 cDNA RNA , RNase RNase . RNA RNase , disposable .*21 RNA .*31 Random primer .*41 RNA . MMLV RTase 42 C RNA PrimeScript RTase.
3 [ ] Code 1st strand cDNA 6210A PrimeScript II 1st strand cDNA Synthesis Kit 50 RT PCR kit RR014A PrimeScript RT-PCR Kit 50 RR055A PrimeScript One Step RT-PCR Kit 50 Full-length cDNA 634925 SMARTer PCR cDNA Synthesis Kit 10 2680A PrimeScript Reverse Transcriptase 10,000 ULicense Notice [K42] [L1] [L15] [M57]
