Transcription of cDNA 합성 - TaKaRa CMS
1 RR037 RR036 primescript RT reagent Kit (RR037) - RT (oligo-dT random hexamer)- Dual-Labeled Probe 5 real time PCR (Step 2)cDNA real time RT-PCR cDNA primescript RT reagent Kit ( perfect real time ) (Code RR037A/RR037B) real time RT-PCR 5x premix cDNA primescript RT Master Mix ( perfect real time ) (Code RR036A/RR036B) primescript RTase 15 1 pg~1 g total RNA (10 ). linearity . 2 (oligo-dT, random hexamer) real time PCR cDNA RR037 RR036 oligo-dT random hexamer cDNA . Poly A poly A real time PCR . RR037 Dual-Labeled Probe . linearity < > primescript RT reagent Kit A , B cDNA , real time PCR standard curve linearity.
2 < > primescript RT reagent Kit , standard curve linearity . (20 )- : total RNA 2 pg~2 , real time PCR (25 )- real time PCR : SYBR premix Ex Taq II ( perfect real time )- : 2 - : Rps18- : perfect real time Support System - : Thermal Cycler Dice real time System A B Tip ! Random primer : Random primer mRNA PCR . random primer . Oligo-dT primer : RNA 3 poly A , oligo-dT primer . poly A poly A PCR primer . polyA PCR 2 mRNA . Gene specific primer : 1-step RT-PCR.
3 , PCR reverse primer .. real time PCR primescript RT Master Mix (RR036)- 5X premix (2 (oligo-dT, random hexamer) )- RNA H2O RT 6 Life Science & Biotechnology 48 DNA real time RT-PCR cDNA primescript RT reagent Kit with gDNA Eraser ( perfect real time ) (Code RR047A / RR047B) RNA gDNA Eraser : DNA DNA : 42 C, 2 , cDNA : 15 DNA real time PCR < > DNA(200ng) total RNA( pg~2 ) primescript RT reagent with gDNA Eraser A , real time PCR . < > DNA total RNA primescript RT reagent Kit with gDNA Eraser gDNA Eraser , DNA . : total RNA ( pg, 20 pg, 200 pg, 2 ng, 20 ng, 200 ng, 2 ) + DNA 200 ng : primescript RT reagent Kit with gDNA Eraser A cDNA real time PCR : SYBR Premix Ex Taq ( perfect real time ) : 2 : Rps18 (102bp) : perfect real time Support System.
4 Thermal Cycler Dice real time System A DNA DNA real time PCR primescript RT reagent Kit ( perfect real time )(Code RR037A)5 primescript Buffer( for real time 1 400 primescript RT Enzyme Mix I 2 100 Oligo dT Primer 50 M 100 Random 6 mers 100 M 400 RNase Free dH2O 1 mlEASY Dilution for real time PCR 1 ml 1 dNTP Mixture & Mg2+ 2 RNase Inhibitor primescript RT Master Mix ( perfect real time )(Code RR036A)5 primescript RT Master Mix perfect real time 1 400 RNase Free dH2O 1 ml 2 EASY Dilution for real time PCR 1 ml 1 primescript RTase RNase Inhibitor Oligo dT Primer Random hexamers dNTP Mixture buffer ( Mg2 ) . primescript RT reagent Kit with gDNA Eraser ( perfect real time ) (Code RR047A) gDNA Eraser 100 5 gDNA Eraser Buffer 200 5 primescript Buffer 2 (for real time ) 1 400 primescript RT Enzyme Mix I 2 100 RT Primer Mix*3 400 RNase Free dH2O 1 ml 2 EASY Dilution (for real time PCR) 1 ml 1 dNTP Mixture 2 RNase Inhibitor * 3.)
5 Oligo dT Primer, Random hexamers cDNA Ovation Pico WTA SystemOvation PicoSL WTA SystemOvation FFPE WTA SystemWT-Ovation One-Direct System total RNA (10 pg ) real time PCR Fragmentation LabelingcDNA Ovation Pico WTAO vation PicoSL WTAWT-Ovation FFPE WT-Ovation One-Direct Encore Biotin ModuleEncore BiotinlL ModuleMicroarray FFPE, LCM, , , , FNA NuGEN SPIA(Single Primer Isothermal Amplification) , representation.
