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Real Time PCR의용도및정량원리 - TaKaRa CMS

2 Life Science & Biotechnology 48 real time PCR PCR , PCR , . , PCR , mRNA SNP . real time PCR real time PCR SNP , (GMO) , , .. real time PCR , , PCR real time PCR . real time PCR real time PCR PCR.

2 · Life Science & Biotechnology 48 Real Time PCRPCR 증폭 산물을 실시간으로 모니터링하는 해석 방법 으로, 기존의 PCR법으로는 …

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Transcription of Real Time PCR의용도및정량원리 - TaKaRa CMS

1 2 Life Science & Biotechnology 48 real time PCR PCR , PCR , . , PCR , mRNA SNP . real time PCR real time PCR SNP , (GMO) , , .. real time PCR , , PCR real time PCR . real time PCR real time PCR PCR.

2 End-point RT-PCR .. PCR 1 cycle DNA 2 cycle DNA plateau .. PCR . 1. real time PCR threshold , ( ). , serial dilution sample real time PCR , . , , Ct (Threshold Cycle) ( ). Ct , Ct ( ).

3 real time PCR real time PCR PCR . intercalator probe . (1) Intercalator (SYBR Green I) intercalator SYBR Green I . SYBR Green I PCR double strand DNA . PCR . 2. Intercalator real time PCR (2) Probe Probe Dual-Labeled Probe Dual-Labeled Probe 5 end , 3 end quencher tag . Dual-Labeled Probe 1 Dual-Labeled Probe.

4 Dual-Labeled Probe annealing hybridization , 3 end quencher . extention real time PCR Taq DNA Polymerase 5 3 exonuclease , Dual-Labeled Probe quencher .. probe Cycling Probe , Molecular Beacon Probe . 3. Dual-Labeled Probe real time PCR real time PCR real time PCR / RT-PCRReal time PCR Product Information VOL 3_1 2 real time PCR Eco57 l PCR-RFLP Cycle Tip! Standard sample ( ).

5 Ct , PCR cycle .1. real time PCR real time PCR SNP typing, (GMO) , , Copy . real time PCR .. real time PCR , , contamination . PCR RealTime PCR . real time PCR Contamination real time PCR Contamination Broad dynamic range.

6 2. real time PCR real time PCR PCR . end-point RT-PCR ..PCR 1 cycle DNA 2 cycle DNA plateau . DNA . DNA ( ) , signal . PCR signal plateau . DNA , . , standardsample real time PCR , DNA.

7 , (threshold) , Ct (Threshold Cycle) . Ct , . standard sample Ct ..1 real time PCR SNP typing Cycling Probe PCR ROX ChannelFAM Channel Signal mutant homo mutant homoEco57 l:CTGAAG(N)16 GACTTC(N)14 wild type : mutant type : PCR-RFLP Sequencing FAM ChannelDNA ConcentrationBaselineFluorescence Detection mutant wild SampleCopy Threshold Log concentrationCycle Ct 012334 PCR Product Information VOL 3_1 2 real time PCR Eco57 l PCR-RFLP Cycle Tip!

8 Standard sample ( ).Ct , PCR cycle .1. real time PCR real time PCR SNP typing, (GMO) , , Copy . real time PCR .. real time PCR , , contamination . PCR RealTime PCR . real time PCR Contamination real time PCR Contamination Broad dynamic range.

9 2. real time PCR real time PCR PCR . end-point RT-PCR ..PCR 1 cycle DNA 2 cycle DNA plateau . DNA . DNA ( ) , signal . PCR signal plateau . DNA , . , standardsample real time PCR , DNA.

10 , (threshold) , Ct (Threshold Cycle) . Ct , . standard sample Ct ..1 real time PCR SNP typing Cycling Probe PCR ROX ChannelFAM Channel Signal mutant homo mutant homoEco57 l:CTGAAG(N)16 GACTTC(N)14 wild type : mutant type : PCR-RFLP Sequencing FAM ChannelDNA ConcentrationBaselineFluorescence Detection mutant wild SampleCopy Threshold Log concentrationCycle Ct 012334 PCR Product Information VOL 3_1 2 real time PCR Eco57 l PCR-RFLP Cycle Tip!


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