Transcription of Gitanjali Multani; Priyanka Multani S0525
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Gitanjali Multani; Priyanka Multani S0525
CALIFORNIA STATE SCIENCE FAIR2016 PROJECT SUMMARYAp2/16 Name(s)Project NumberProject TitleAbstractSummary StatementHelp ReceivedGitanjali Multani ; Priyanka MultaniEarly Detection of Epithelial Ovarian Cancer via B7-H4 Quantificationin a Microfluidic SystemS0525 Objectives/GoalsIt has recently been discovered that B7-H4 is a protein in only low amounts in normal tissues but highlyconcentrated in over 90% of cases. Utilizing the monoclonal antibody, MIH43, which recognizes B7-H4,a method is presented to ascertain the presence of B7-H4 through nanoparticle luminescence, through theuse of a biotinylated antibody in sandwich ELISA. Our goal is to compact the system into a , EDC and Sulfo NHS crosslinkers achieve the direct MIH43 conjugation to an 50nm iron oxidenanoparticle via carbohydrate moiety. A subsequent sandwich ELISA test employs avidin-horseradishperoxidase (HRP) enzyme to stimulate the luminescence of a biotinylated detection antibody, allowing thedetermination of the concentration of B7-H4 that bound to the particle complex.
Gitanjali Multani; Priyanka Multani Early Detection of Epithelial Ovarian Cancer via B7-H4 Quantification in a Microfluidic System S0525 Objectives/Goals It has recently been discovered that B7-H4 is a protein in only low amounts in normal tissues but highly concentrated in over 90% of cases. Utilizing the monoclonal antibody, MIH43, which ...
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