Transcription of GUIDELINES FOR HEMATOXYLIN & EOSIN STAINING
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GUIDELINES FOR. HEMATOXYLIN & EOSIN . STAINING . National Society for Histotechnology 4201 Northview Drive, Suite 502. Bowie, Maryland 20716-2604. Phone: 301-262-6221 Fax: 301-262-9188. Email: Website: -1- GUIDELINES for HEMATOXYLIN and EOSIN STAINING The desired end result of a tissue stained with HEMATOXYLIN and EOSIN (H&E) is based upon what seems to be almost infinite factors. Pathologists or the diagnostician have individual preferences for section thickness, intensities, and shades. The choice of which reagents to use must take into consideration: cost, method of STAINING (manual vs. automatic), option of purchasing commercially-prepared or technician-prepared reagents, safety, administration policies, convenience, availability, quality, technical limitations, as well as personal preference.
Bluing reagents change the reddish – purple hematoxylin to a blue or purple blue color. It is a pH dependent reaction and occurs in an alkaline solution. a. Troubleshooting. Reddish color of a stained section is due to inadequate bluing. Bluing reagents should have a pH of approximately 8. It is not possible to over-blue a section.
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