Transcription of HPLC METHOD DEVELOPMENT AND VALIDATION: AN …
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HPLC METHOD DEVELOPMENT AND VALIDATION: AN …
274 Santhosh al. / Vol 4 / Issue 4 / 2014 / 274-280. HPLC METHOD DEVELOPMENT AND VALIDATION: AN OVERVIEW G. Santhosh*, , , Maheswara Rao Department of Pharmaceutical Analysis and Quality Assurance, CMR College of Pharmacy, Medchal Road, Kandlaykoya, Hyderabad, India. ABSTRACT High performance liquid chromatography (HPLC) is an analytical technique which is proficient to separate, detect and quantify various drugs and its related degradants. An appropriate mobile phase, column, column temperature, wavelength and gradient must be found that affords suitable compatibility and stability of drug as well as degradants and impurities.
accuracy, precision, and convenience of the final method [8]. Step 2 - Selection of initial conditions This step determines the optimum conditions to adequately retain all analytes; that is, ensures no analyte has a capacity factor of less than 0.5 (poor retention could result in peak overlapping) and no analyte has a capacity
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