Transcription of Microbial growth kinetics and fermentation
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Microbial growth kinetics and fermentation
Microbial growth kinetics and fermentation Measurement of Microbial growth 3. Measurement of Microbial growth A. Measurement of cell numbers microscopic observation on specially etched slides (hemacytometers). cell counts involve plating diluted samples (using a pour plate or spread plate). B. Measurement of cell mass-may be used to approximate the number of microorganisms (Suitable parameters may be dry weight, light scattering in liquid solutions, or biochemical determinations of specific cellular constituents such as protein, DNA). If plated on a suitable medium, each viable unit grows and forms a colony. Each colony that can be counted is called a colony forming unit (cfu) and the number of cfu's is related to the viable number of bacteria in the sample 1 OD600 nm = 109 cells/ml (E. coli Bacterial colonies growing on a plate of nutrient agar.)
– growth optimum between pH 0 and pH 5.5 • Neutrophiles – growth optimum between pH 5.5 and pH 7 • Alkalophiles – growth optimum between pH 8.5 and pH 11.5 • most acidophiles and alkalophiles maintain an internal pH near neutrality – The plasma membrane is impermeable to protons • some synthesize proteins that provide protection
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