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HPLC Standard Operating Procedure

HPLC Standard Operating Procedure Last Revised March, 3, 2016 This document is intended to be a guide for Operating the Garner Lab s HPLC systems and is only intended to provide instructions for users who are already generally familiar with HPLC operation. If you ve never used an HPLC before, then you must be trained by a qualified senior member of the Garner Research Group. 1. System Start-up HPLC 1) Turn on the UV detector for the HPLC you plan to use at least 10 minutes before running samples. The power switch is located on the front of the detector. o If the detector is already on, but the UV lamp is off, turn the lamp on by pressing Shift + Lamp two times. o If the System is not calibrated message is displayed, then the system must be recalibrated as follows: o Ensure that the flow cell contains UV/Vis neutral solvent.

HPLC Standard Operating Procedure Last Revised March, 3, 2016 This document is intended to be a guide for operating the Garner Lab’s HPLC systems and is only intended to provide instructions for users who are already generally familiar with HPLC operation. If

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