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HPLC Troubleshooting Cover

HPLCT roubleshootingGuide 1 HPLC Troubleshooting Uwe D. Neue, Waters Corporation 1. Column Life-Time 2. Variable Retention Times 3. Drifting Retention Times 4. Column-to-Column and Batch-to-Batch Reproducibility 5. Sample Preparation Problems 6. Sources of Peak Tailing 7. Normal-Phase Chromatography 8. System Volume, Dead Volume, Dwell Volume 9. Transfer of Gradient Methods 10. Clogged System 11. Column Plate-Count 12. Column Backpressure 13. Peak Area Fluctuations 14. Ghost Peaks 15. Dependence of Retention Times on pH 16.

phase or they may be strongly adsorbed. As you inject more and more samples, these contaminants build up on the top of the column and prevent the sample to properly adsorb and distribute. This results in a distortion of the peak profile. Often this problem is accompanied by an increase in back pressure. Q.: OK, that could be it.

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