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HPLC Troubleshooting Guide

North Harrison Road, Bellefonte, PA 16823-0048 USATel: (800) 247-6628 (814) 359-3441 Fax: (800) 447-3044 (814) 826 EHPLC Troubleshooting GuideHow to identify, isolate, and correct the most common HPLC problemsCONTENTS:Isolating HPLC Problems .. 2 How to Prevent Mobile Phase Problems .. 2 Isolating Pump Problems .. 3 Injector and Injection Solvents .. 3 Column Protection .. 3 Getting the Most from Your Analytical Column .. 3 Solving Detector Problems .. 4 Column Heater, Recorder .. 4 Keeping Accurate Records .. 4 Problem Index .. 4 HPLC Problems, Causes and Remedies .. 5-13 Restoring Your Column's Performance .. 14-15 Preventing and Solving Common Fitting Problems .. 16A Selection of Column Protection Products.

Recycling the mobile phase used for isocratic separations has become more popular in recent years as a means of reducing the cost of solvents, their disposal, and mobile phase preparation time. ... The pump must deliver a constant flow of solvent to the column over a wide range of conditions. Modern HPLC pumps incorpor-ate single or dual piston ...

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