Transcription of HUMAN PLASMA FOR FRACTIONATION - …
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EUROPEAN PHARMACOPOEIA PLASMA for fractionationwith a mixture of 19 volumes ofglacial acetic acid Rand81 volumes ofmethanol at 600 nm in an instrument having a linear responseover the range of measurement. Calculate the result as themean of 3 measurements of each suitability: in the electropherogram obtained withthe reference solution on cellulose acetate or on agarosegels, the proportion of protein in the principal band is withinthe limits stated in the leaflet accompanying the : in the electropherogram obtained with the testsolution on cellulose acetate or on agarose gels, not morethan 5 per cent of protein has a mobility different from thatof the principal band. This limit is not applicable if albuminhas been added to the preparation as a stabiliser; for suchpreparations, a test for protein composition is carried outduring manufacture before addition of the of molecular size. Liquid chromatography( ).Test solution.
EUROPEAN PHARMACOPOEIA 6.0 Human plasma for fractionation with a mixture of 19 volumes ofglacial acetic acid R and 81 volumes of methanol R.Measuretheabsorbanceofthe bands at 600 nm in an instrument having a linear response over the range of measurement.
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