Transcription of Immunocytochemistry (ICC)
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Immunocytochemistry (ICC)
Learn more | more | (ICC) HandbookConjugateExcitation/EmissionEmis sionColorLaser (Excitation Source)NotesDyLightTM 405400/420 VioletViolet (405 nm)Bright and photostableAlexa Fluor 405401/421 VioletViolet (405 nm)Best when used with more abundant targetsDyLightTM 350353/432 Violet-BlueUltraviolet (355 nm)Can be used with DyLightTM 488, 594 and 647 in multiplexingAlexa Fluor 350346/442 Violet-BlueUltraviolet (355 nm)Often used with Alexa Fluor 488, 594 and 647 in multiplexing, best for high-abundance targetsDyLightTM 488493/518 GreenBlue (488) Brighter, photostable replacement for FITC; not suitable for use with GFPA lexa Fluor 488495/519 GreenBlue (488) Photostable over a broad pH range; replaces FITCFITC495/519 GreenBlue (488) Small organic fluorophore; cannot be used with DyLightTM 488, Alexa Fluor 488 or GFPDyLightTM 405LS397/572 YellowViolet (405 nm)Superior alternative to Pacific Orange; good choice for multicolor applications on the violet laserAlexa Fluor 546556/573 YellowYellow-Green (561 nm)Photostable over a broad pH range; brighter than Cy3 DyLightTM 550562/576 YellowYellow-Green (561 nm)PE565/578 YellowYellow-Green (561 nm)Subject to photobleaching; can be excited by the 488, 532, and 561nm lasers on flow cytometersTexas Red 595/613 OrangeYellow-Green (561 nm)Very bright fluorescence; use a
the filter name. In addition, a rough guide for laser/filter selection is provided by the number listed after some dyes, such as 488 in Alexa Fluor® 488 or DyLightTM 488. Extinction coefficients (ε) and brightness of fluorochrome The brightness of fluorochromes is directly proportional to the extinction coefficient,
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