Transcription of InstaGeneTM Matrix - Bio-Rad
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InstaGeneTM Matrix - Bio-Rad
InstaGeneTMMatrixCatalog # 03/17/2000 3:43 PM Page 2 Table of ContentsSection 1. Contents and 2. Instruction for DNA Preparation From Whole DNA Preparation From Cultured Mammalian DNA Preparation From Bacteria .. 03/17/2000 3:43 PM Page 31 Section 1. Contents and StorageThis bottle contains 20 ml of 6% InstaGene Matrix and a magneticstirbar. This is sufficient for 100 DNA preparations. Upon arrival, storethe Matrix at 4 WarningAvoid prolonged exposure of the Matrix to UV UseInstaGene Matrix allows fast and easy preparation of PCR*amplifiable DNA by eliminating labor intensive phenol/chloroformextraction steps.
3 2.2 DNA Preparation From Cultured Mammalian Cells 1 Pellet 200 µl of cell suspension from media in a microfuge tube. Spin at 10,000–12,000 rpm for 1 minute and remove the supernatant. 2 Resuspend cells in 1 ml of 1x PBS and centrifuge for 1 minute at 10,000–12,000 rpm. 3 Resuspend cells in autoclaved water at 20–30 cells/µl. 4 Add 20 µl of this cell suspension to 200 µl of ...
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