Transcription of Introduction to Small-Angle X-ray Scattering
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Introduction to Small-Angle X-ray Scattering
Introduction to Small-Angle X-ray Scattering Thomas M. Weiss Stanford University, SSRL/SLAC, BioSAXS beamline BL 4-2 BioSAXS Workshop, March 28-30, 2016. Sizes and Techniques Diffraction and Scattering Scattering of X-rays from a single electron Ie Thomson formula for the scattered intensity from a single electron 2 . I0. 1 cos( 2 ) 1. I e r0. 2. 2. I0. 2 r 2. e 15. r0 2. 2 . 817 10 m I 0 : Intensity of incoming X - rays mc 2 : angle of observatio n I e : Intensity of scattered X - rays Classical electron radius The Thomson formula plays a central role for all Scattering calculations involving absolute intensities.
Putnam et al., Quat.Rev.Bioph 40,3 (2007), Fig24 Hiller et al., Biomaterials, 24 (2003), Fig5 . ab-initio structure determination • using spherical harmonics to produce molecular envelopes that fit the experimental scattering data Envelop models • fitting the scattering data using bead as
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