Transcription of Introduction to Spectrophotometry
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Introduction to Spectrophotometry
1 Introduction to Spectrophotometry Objective: To learn how to use a spectrophotometer. Materials: UV/visible spectrophotometer TruView Cuvette Polystyrene cuvettes Background Biophotonics is the study of the interaction of biological materials with light and other forms of radiant energy. Radiation is energy that comes from a source and can travel through material or space. The following illustration is of the electromagnetic spectrum along with various wavelengths. 2 Tissue and cells are composed of a variety of organic compounds (DNA, proteins, lipids, and carbohydrates). Light interacts with these molecules in different ways: reflection, absorption, transmission, and scattering.
A Hemocytometer is used to count the number of cells in a given volume. The problem with using a Hemocytometer is that the total number of bacteria is determined not the number of bacteria which are alive. The second method used to determine bacterial concentration is …
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