iScript cDNA Synthesis Kit - Bio-Rad Laboratories

Component Volume per Reaction, l 5x iScript Reaction Mix 4 iScript Reverse Transcriptase 1 Nuclease-free water Variable RNA template (100 fg 1 g total RNA)* Variable Total volume 20 * When using larger amounts of input RNA (>1 g), the reaction should be scaled up (for example, 40 l reaction for 2 g, or 100 l reaction for 5 g) to ensure optimum Synthesis ProtocolIncubate the complete reaction mix in a thermal cycler using the following protocol: Priming 5 min at 25 C Reverse transcription 20 min at 46 C RT inactivation 1 min at 95 C Optional step Hold at 4 C Recommendation for Optimal Results Using the iScript cDNA Synthesis KitThe maximum amount of the cDNA reaction that is recommended for downstream PCR is one-tenth of the reaction volume, typically 2 ProductsCatalog #DescriptionReverse Transcription Reagents for Real-Time qPCR170 8 8 4 0iScript Reverse Transcription Supermix for RT-qPCR17250 37iScript Advanced cDNA Synthesis Kit for RT-qPCR170 8 8 9 6iScript Select cDNA Synthesis Kit17250 3 4iScript gDNA Clear cDNA Synthesis KitReagents for Real-Time qPCR1725270 SsoAdvanced Universal SYBR Green Supermix 1725280 SsoAdvanced Universal Probes Supermix1725120i Taq Universal SYBR Green Supermix 172513 0iTaq Universal Probes Su

ComponentIntroduction Volume per Reaction, µl 5x iScript Reaction Mix 4 iScript Reverse Transcriptase 1 Nuclease-free waterPCR (RT-qPCR). This kit …

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