Transcription of iScript cDNA Synthesis Kit - Bio-Rad Laboratories
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iScript cDNA Synthesis Kit - Bio-Rad Laboratories
Component Volume per Reaction, l 5x iScript Reaction Mix 4 iScript Reverse Transcriptase 1 Nuclease-free water Variable RNA template (100 fg 1 g total RNA)* Variable Total volume 20 * When using larger amounts of input RNA (>1 g), the reaction should be scaled up (for example, 40 l reaction for 2 g, or 100 l reaction for 5 g) to ensure optimum Synthesis ProtocolIncubate the complete reaction mix in a thermal cycler using the following protocol: Priming 5 min at 25 C Reverse transcription 20 min at 46 C RT inactivation 1 min at 95 C Optional step Hold at 4 C Recommendation for Optimal Results Using the iScript cDNA Synthesis KitThe maximum amount of the cDNA reaction that is recommended for downstream PCR is one-tenth of the reaction volume, typically 2 ProductsCatalog #DescriptionReverse Transcription Reagents for Real-Time qPCR170 8 8 4 0iScript Reverse Transcription Supermix for R
ComponentIntroduction Volume per Reaction, µl 5x iScript Reaction Mix 4 iScript Reverse Transcriptase 1 Nuclease-free waterPCR (RT-qPCR). This kit …
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