Transcription of ISP Medium No. 3 - HiMedia Labs
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ISP Medium No. 3 - HiMedia Labs
ISP Medium No. 3 M358. ISP Medium No. 3 is used for the cultivation and characterization of Streptomyces species as per International Streptomyces Project. Composition**. Ingredients Gms / Litre Oat Meal Agar Trace salts, - Ferric sulphate heptahydrate Manganese chloride tetrahydrate Zinc sulphate heptahydrate Final pH ( at 25 C) **Formula adjusted, standardized to suit performance parameters Directions Suspend 38 grams(equivalent weight of dehydrated Medium per litre) in 1000 ml distilled water. Heat to boiling to dissolve the Medium completely. Sterilize by autoclaving at15 lbs pressure (121 C) for 15 minutes. Cool to 45-50 C and mix intermittently while pouring into sterile Petri plates for even distribution of oatmeal. Principle And Interpretation ISP Medium No. 3 is formulated based on the original formula of Shirling and Gottleib (1). The Medium is often referred to as Oat Meal Agar.
Please refer disclaimer Overleaf. ISP Medium No. 3 M358 ISP Medium No. 3 is used for the cultivation and characterization of Streptomyces species as per International
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