Transcription of ISP Medium No. 5 (Glycerol Asparagine Agar Base)
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ISP Medium No. 5 (Glycerol Asparagine Agar Base)
ISP Medium No. 5 ( glycerol Asparagine Agar Base) M360. ISP Medium No. 5 ( glycerol Asparagine Agar Base) is recommended for cultivation of Streptomyces species as per International Streptomyces Project. Composition**. Ingredients Gms / Litre L- Asparagine Dipotassium phosphate *Trace salt solution (ml) Agar 1ml of Trace salt solution contains - Ferrous sulphate heptahydrate Manganese chloride tetrahydrate Zinc sulphate heptahydrate Final pH ( at 25 C) **Formula adjusted, standardized to suit performance parameters Directions Suspend grams in 1000 ml distilled water containing 10 ml glycerol . Heat to boiling to dissolve the Medium completely. Sterilize by autoclaving at 15 lbs pressure (121 C) for 15 minutes. Mix well and pour into sterile Petri plates. Principle And Interpretation ISP Medium No. 5 ( glycerol Asparagine Agar Base) is based on the formulation described by Shirling and Gottlieb (1) and is used for cultivation and characterization of Streptomyces species as recommended by the International Streptomyces Project.
Please refer disclaimer Overleaf. ISP Medium No. 5 (Glycerol Asparagine Agar Base) M360 ISP Medium No. 5 (Glycerol Asparagine Agar Base) is recommended for cultivation of Streptomyces species as per
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