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Laboratory Procedure Manual

Laboratory Procedure Manual Analyte: Biochemistry Profile Matrix: Serum Method: Hitachi Model 917 Multichannel Analyzer Method No.: Revised: as performed by: Coulston Foundation Alamogordo, New Mexico Contact: Ms. Love Julian Biochemistry Profile in Refrigerated Serum NHANES 1999 2000. Public Release Data Set Information This document details the Lab Protocol for NHANES 1999 2000 data. Two laboratories performed this testing during 1999 2000. To maintain confidentiality of the participants, the quality control summary statistics and graphs were combined to mask the individual analysis dates from the two laboratories. Methods for both labs are included in this release. A tabular list of the released analytes follows: Lab Number Analyte SAS Label (and SI units). LBXSAL Albumin (g/dL). LBDSALSI Albumin (g/L). LBXSATSI ALT (U/L). LBXSASSI AST (U/L). LBDSAPSI Alkaline phosphatase (U/L). LBXSBU Blood urea nitrogen (mg/dL).

International Federation of Clinical Chemistry (IFCC) has now recommended standardized procedures for ALT determination, including 1) optimization of substrate concentrations, 2) the use of Tris buffers, 3) preincubation of a combined buffer and serum solution to allow side reactions with NADH to occur,

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