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Laboratory Procedure Manual

Laboratory Procedure Manual Analyte: Total cholesterol , HDL- cholesterol , Triglycerides, and LDL- cholesterol Matrix: Serum Method: Hitachi 704 Analyzer which is serviced by Roche Diagnostics (formerly Boehringer-Mannheim Diagnostics), Indianapolis as performed by: Lipid Laboratory Johns Hopkins University School of Medicine Lipoprotein Analytical Laboratory 600 North Wolfe Street Blalock 1379 Baltimore, MD 21287 410-614-1030 Contact: Peter O. Kwiterovich, Jr., Total cholesterol , Direct HDL, Precipitated HDL, Triglycerides, and LDL NHANES 2003-2004 Public Release Data Set Information This document details the Lab Protocol for NHANES 2003-2004 data. A tabular list of the released analytes follows: Lab Number Analyte SAS Label (and SI units) LBXTC Total cholesterol (mg/dL) LBDTCSI Total cholesterol (mmol/L) LBXHDD HDL- cholesterol (mg/dL) LBDHDDSI HDL- cholesterol (mmol) LBXTR Triglycerides(mg/dL) l13_c LBDTRSI Triglycerides(mmol/L Page 2 of 23 Total cholesterol , Direct HDL, Precipitated HDL, Triglycerides, and LDL NHANES 2003-2004 1.)

LDL-cholesterol is calculated from measured values of total cholesterol, triglycerides and HDL-cholesterol according to the relationship: [LDL-chol] = [total chol] - [HDL-chol] - [TG]/5 where [TG]/5 is an estimate of VLDL-cholesterol and all values are expressed in mg/dL.

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