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Legionella Sampling Procedure and Potential Sampling Sites

Centers for Disease Control and Prevention Sampling Procedure and Potential Sampling Sites Protocol for collecting environmental samples for Legionella culture during a cluster or outbreak investigation or when cases of disease may be associated with a facility. Sampling should only be performed after a thorough environmental assessment has been done and a Sampling plan has been made. This protocol describes how to take standard biofilm swab, bulk water, and filter samples from commonly sampled Sites . This protocol may be used in conjunction with the following tools: Legionella ENVIRONMENTAL ASSESSMENT FORM. SAMPLE DATA SHEET. LEGIONELLOSIS OUTBREAK INVESTIGATION VIDEOS: Legionella Ecology and an Introduction to Environmental Health and Engineering Conducting and Interpreting the Environmental Assessment How to Make a Sampling Plan How to Sample Potable Water How to Sample Cooling Towers How to Sample Spas and Fountains MATERIALS NEEDED.

Sterile plastic 500 mL or 1 L bottle for testing chlorine level, pH, and temperature. pH test kit. Chlorine test kit sensitive enough to detect chlorine level below 2 ppm and up to 10 ppm (may need two kits). Free chlorine may be measured when it is known that chlorine is the method of disinfection (as opposed to

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