Transcription of Matrix-Assisted Laser Desorption/Ionization (MALDI)
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Matrix-Assisted Laser Desorption/Ionization (MALDI)
UC Berkeley College of ChemistryChemistry 105 Instrumental Methods in Analytical ChemistryMatrix- assisted LaserDesorption/ ionization ( maldi )Final PaperAuthor:JonathanMelvilleGraduate Student Instructors:RichardCooper& DanielMortensenMay 9, 2014 Contents1 Introduction12 Cocrystal Formation .. Laser Excitation, Ablation, and Analyte ionization .. Mass Spectrometry ..63 Proven Peptide Mass Fingerprinting .. Polymer Molar Mass Distribution ..94 Competition105 Outlook126 Conclusion12 Bibliography131 IntroductionMALDI, or Matrix-Assisted Laser Desorption/Ionization , is an analytical technique usedfor soft ionization ( ionization without fragmentation) of particles for use in mass spec-trometry, especially biopolymers or other macromolecules that are difficult to ionize with-out fragmentation. By irradiating a sample with a high-energy (usually UV) Laser pulse,a plume of ions can be produced that can be analyzed (this is known as Laser Desorp-tion ionization ).
MALDI, or Matrix-Assisted Laser Desorption/Ionization, is an analytical technique used for soft ionization (ionization without fragmentation) of particles for use in mass spec-trometry, especially biopolymers or other macromolecules that are di cult to ionize with-out fragmentation. By irradiating a sample with a high-energy (usually UV) laser ...
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