Transcription of Matrix-Assisted Laser Desorption/Ionization (MALDI)
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Matrix-Assisted Laser Desorption/Ionization (MALDI)
UC Berkeley College of ChemistryChemistry 105 Instrumental Methods in Analytical ChemistryMatrix- assisted LaserDesorption/ ionization ( maldi )Final PaperAuthor:JonathanMelvilleGraduate Student Instructors:RichardCooper& DanielMortensenMay 9, 2014 Contents1 Introduction12 Cocrystal Formation .. Laser Excitation, Ablation, and Analyte ionization .. Mass Spectrometry ..63 Proven Peptide Mass Fingerprinting .. Polymer Molar Mass Distribution ..94 Competition105 Outlook126 Conclusion12 Bibliography131 IntroductionMALDI, or Matrix-Assisted Laser Desorption/Ionization , is an analytical technique usedfor soft ionization ( ionization without fragmentation) of particles for use in mass spec-trometry, especially biopolymers or other macromolecules that are difficult to ionize with-out fragmentation.
(SDS-PAGE) and gel permeation chromatography (GPC)). The authors supposed that this phenomenon was due to the unique qualities of the cobalt nanocrystals (low speci c 2. heat, high photoabsorptivity, and large surface area per volume) that enhanced the speed
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