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METHOD 8327 - US EPA

SW-846 Update VII 8327- 1 Revision 0 July 2021 METHOD 8327 PER- AND POLYFLUOROALKYL SUBSTANCES (PFAS) BY LIQUID CHROMATOGRAPHY/TANDEM MASS SPECTROMETRY (LC/MS/MS) TABLE OF CONTENTS SCOPE AND 2 SUMMARY OF METHOD .. 5 DEFINITIONS .. 5 INTERFERENCES .. 5 SAFETY .. 7 EQUIPMENT AND SUPPLIES .. 7 REAGENTS AND STANDARDS .. 9 SAMPLE COLLECTION, PRESERVATION, AND STORAGE .. 13 QUALITY CONTROL .. 14 CALIBRATION AND STANDARDIZATION .. 20 PROCEDURE .. 20 DATA ANALYSIS AND CALCULATIONS .. 25 METHOD PERFORMANCE .. 26 POLLUTION PREVENTION .. 26 WASTE 26 REFERENCES .. 27 TABLES, DIAGRAMS, FLOW CHARTS, AND VALIDATION DATA .. 28 TABLE 1. RECOVERY AND precision OF TARGET ANALYTES IN MULTI-LABORATORY STUDY MATRICES PREPARED BY METHOD 3512 .. 29 TABLE 2. RECOMMENDED PRECURSOR IONS AND PRODUCT IONS AND EXAMPLE CHROMATOGRAPHIC RETENTION TIMES.

precision and bias. 4.3.1 All solvents should be LC/MS gradeor equivalent, , to minimize interference problems. Solvents must be checked by lot prior to use. 4.3.2 PFAS contamination has been found in reagents, glassware, tubing, polytetrafluoroethylene (PTFE) LC vial caps, disposable pipets, aluminum foil, glass

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