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Method Development Guide (rev. 05/04) - ZirChrom

ZirChrom -EZ & ZirChrom -MSMethodDevelopmentGuidetel1-8866-SSTAB LE-11/fax1-7763-4421-22319 -EZ & ZirChrom -MSMethodDevelopmentGuideColumn Use TipsLewisAcidSite Deactivated Zirconia-BBasedColumnsforHPLCI ncludesNEWQ uick-Start Guide !PART I / QUICK-SSTARTGUIDES tart-uupProcedureX2 InstallingtheColumnValidatingColumnPerfo rmanceMobilePhasepHEquilibratingtheColum nUsewithLC/MSZirChrom -EEZ& ZirChrom -MMSX4 GeneralUseTipsColumnMaintenanceX5 Cleaning/RegenerationProcedureStorageCon ditionsPART you for purchasing a ZirChrom HPLC column. Due to its unique characteristics namely that its packing material is zirconia-based rather than silica-based, we strongly recommend that you read this Guide beforeusing your column.

USE WITH LC/MS We recommend running at least two 30-minute gradients of 10-90% acetonitrile/water (weak to strong) to condition your column before use with your HPLC/MS system. Quick-Start ... Method Development Guide (rev. 05/04) ...

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