Transcription of Method for the Determination of b-Carotene in Supplements ...
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Method for the Determination of b-Carotene in Supplements ...
DIETARY SUPPLEMENTSM ethod for the Determination of b-Carotene in Supplements andRaw Materials by Reversed-Phase Liquid Chromatography:Single Laboratory ValidationJOSEPH SCHIERLE, BERND PIETSCH, ALAN CERESA, and CHRISTIAN FIZETDSM Nutritional Products Ltd, Research and Development, Analytical Research Center, PO Box 3255, CH-4002 Basel,SwitzerlandEDWARD H. WAYSEKC aravan Products Co. Inc., 100 Adams Dr, Totowa, NJ 07512A single laboratory validation (SLV) study wasconducted for a liquid chromatography (LC) Method for the Determination of total andall-trans- b-Carotene in a variety of dietarysupplements, including multivitamin tablets,softgels, capsules, and beadlet raw variants were developed for the different types of Supplements tested based upon thesupplement type and level of b-Carotene . Waterdispersible formulations such as powders,emulsions, tablets, and capsules wereenzymatically digested with protease and extracted with dichloromethane ethanol.
for the analysis of b-carotene in commercial product forms. Schierle et al. (4) re porte d a spe ctrophotom etric pr ocedure for the mination of total b-carotene in food additives with varying cis-/trans-ratios using an isobestic wavelength. However, spectrophotometric methods cannot differentiate between
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