Laboratory Diagnosis of Hemoglobinopathies and Thalassemia

Diagnosis • Indications for Testing – Hemolytic anemia; family history of hemoglobinopathy • Laboratory Testing – Initial testing – CBC with peripheral smear – Polychromasia, spherocytes, schistocytes, sickle cells, Heinz bodies, basophilic stippling; however, the lack of any of these cells does not rule out hemolytic anemia

  Laboratory, Diagnosis, Laboratory diagnosis of

Lymphoma Diagnostic Work-up from a Lab Perspective

Non-Hodgkin lymphoma (NHL) 2. Hodgkin (disease) lymphoma 3. Multiple myeloma Lymphomas are solid tumors of the hematopoietic system. Neoplasms of lymphoid origin, typically causing lymphadenopathy

  Neoplasm, Lymphoma, Hodgkin, Lymphoid, Hodgkin lymphoma

Resolution of ABO Discrepancies

4+0 A1 Cell B Cell 1+ Lectin Patient’s Cells D. biflorus 0 Antibody Screen I.S. (RT) AHG Check Cells Screening Cell 1 0 0 Screening Cell 2 0 0 Screening Cell 3 0 0 Auto Control 0 0 Forward Type Reverse Type Most Anti-A reagents react strongly with A2 Cells Unexpected Reactivity with A1 Cells No unexpected antibodies detected

  Creativity

Cytogenetics Lecture 1: Introduction to Clinical Cytogenetics

Cell Division and The Cell Cycle •Mitosis: division of somatic cells •Meiosis: division of gametic cells •Cell cycle: 4 phases: Mitosis (M), Gap1 (G1), Synthesis (S), Gap2 (G2) NCBI Bookshelf: Cooper, The Cell: A Molecular Approach, 2nd Ed 2000

  Cells, Somatic

HYPOCELLULAR BONE MARROW… WHAT’S NEXT?

DNA damage response pathway 1. CORE COMPLEX: 8 wild type proteins ‘FANC’ (A,B,C,E,F,G,L,M) and 4 additional proteins FAAP (16,20,24,100) 2. ID COMPLEX: two downstream proteins FANC I and D2 3. DOWNSTREAM EFFECTOR COMPLEX: 5 other proteins: FANCD1, BRCA2, FANCJ, BACH1, and BRIP1 21 known FA subtypes 1 •Mutation of …

Kidney Stones: Diagnosis, Treatment, & Future Prevention

Describe the clinical presentation, laboratory, and radiographic findings of an individual affected by a kidney stone. 2. Compare 3 composition types of kidney stones and their clinical management. 3. Differentiate spontaneous and familial risk factors for kidney stone development.

  Presentation

Microscopy of CSF & Body Fluids - University of Utah

Microscopy of CSF & Body Fluids May 13, 2019 Professor of Pathology University of Utah Health Executive Director, Clinical Trials & PharmaDx ARUP Laboratories

Diagnosis of Monoclonal Gammopathies

–Protein Electrophoresis • Serum • Urine –Immunofixation • Free light chain ratio assay Outline. Serum protein electrophoresis (sPEP) •Intended for the separation of human serum proteins •Divides proteins in five major fractions: albumin, alpha-1, alpha-2, beta and gamma .

  Protein, Serum, Electrophoresis, Serum protein electrophoresis, Protein electrophoresis

Analytical Measurement Range: Examples and Approaches ...

accuracy of the instrument or test system measurement response. o CLIA ’88, Sec. 493.2, Definitions ... • Mayyy decrease the lower limit of the analytical measurement range by: ... o May use precision data for control material near the target

  Measurement, Ranges, Lower, Precision, Limits, Accuracy, Measurement range, Lower limit

NanoDrop 2000/2000c Spectrophotometer

Lower precision pipettors (0-10 µL and larger) are not as good at delivering 1 µL volumes to the measurement pedestal. If the user is unsure about the sample characteristics or pipettor accuracy, a 2 µL sample volume is recommended. Pedestal Basic Use 1. Raise the sampling arm and pipette the sample onto the lower measurement pedestal. 2.

  Measurement, Lower, Precision, Accuracy, Lower precision, Lower measurement

The Importance of Temperature Monitoring in your Lab

Note: With accuracy limitations typically >1C, most healthcare (regulated, audited and accredited) applications would not recommend thermocouple technologies. Type/Material Wire Color Common Accuracy Special Accuracy Limit Range Type-T Copper Constantan Blue 1C 0.5C -200C to 350C Type-J Iron Constantan Black 2.2C 1.1C 0C to 780C Type-K

  Limits, Accuracy, Accuracy limit

Methods for Handling Concentration Values Below the Limit ...

Methods for Handling Concentration Values Below the Limit of Quantification in PK Studies James R. Johnson, Summit Analytical, LLC., Cary, NC USA ABSTRACT Bioanalytical assays for drug concentrations are validated measures within a pre-specified range, with the lower end of that range defined as the “lower limit of quantification” (LLOQ).

  Lower, Limits, Lower limit

Metrology - IIT Kanpur

• Metrology is the science of measurement • Dimensional metrology is that branch of Metrology which deals with measurement of “dimensions“ of a part or workpiece (lengths, angles, etc.) • Dimensional measurements at the required level of accuracy are the essential link between the designers’ intent and a delivered product.

  Measurement, Accuracy, Metrology, Measurement of

Components and Methods for Current Measurement

Depending on the transistor product, the accuracy tolerance range can vary from as low as 5 % to as wide as 15 % or 20 %. This is generally not suitable for current control applications that typically require 1 % measurement accuracy, but is intended for overcurrent and short circuit protection. Fig. 7 4. Resistor Technology Options

  Measurement, Accuracy, Measurement accuracy