Transcription of Microfluidics for DNA Analysis
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Microfluidics for DNA Analysis
1EE C245 Microfluidics for DNA AnalysisDr. Thara SrinivasanLecture 19 Picture credit: Nanogen2U. Srinivasan EE C245 Lecture Outline Reading from reader Mastrangelo, C. H. Microfabricated Devices for Genetic Diagnostics, (1998) pp. 1769-87. Khandurina, J. et al., Bioanalysis in microfluidic Devices, (2002) pp. 159-83. Zhang, L., et al., Microchip Electrophoresis-Based Separation of DNA, (2003) pp. 1645-54. Today s Lecture DNA and Analysis Methods Scaling in Microfluidics Survey of Microfabricated Chips23U. Srinivasan EE C245 DNASBP5 3 Genetic information is stored in chromosomes as long strings of DNA grouped as genes In humans, 46 chromosomes are 50 - 400 106 DNA units long (compared to 4 106 for E.)
Microfluidics for DNA Analysis Dr. Thara Srinivasan ... Integrated Microfluidics Mathies et al., Hilton Head 2002 • Mathies group microfabricated 96-channel CE plate with integrated: • Pneumatic valves and pumps ~ PDMS • Resistive heaters and temperature sensors ~ Ti/Pt
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