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Microscopy I Light and Electron Microscopy

Microscopy ILight and Electron MicroscopyReplica of van Leeuwenhoek s(1632-1723) microscope constructed c. 1670. Moody Medical Library, Univ. Texas Medical Branch, Galveston, TXUse the information in this tutorial to supplement the visuals in lab and the information in Chapters 1, 8 and 9 in your lab manual .Replica of Culpepper tripod microscope built c. 1725 by Edmund Culpepper (1670-1738). Collection of Moody Medical Library, Univ. Tex., Galveston, TX. (Replica by Replica Rara Ltd. Antique Microscopes)ContentsI. Types of MicroscopyA. Light Microscopy1. Brightfield2. Darkfield3. Phase Contrast4. Polarizing5. DIC6. laser scanningB. Electron Microscopy1. Scanning2. TransmissionII.

Position of light source and objectives is “inverted”--light source is above specimen and objective lenses are located beneath the stage. Light Objectives The basic design of bright field microscopes has been modified for special uses. Inverted microscopes (right) allow viewing of cells in flasks, welled-plates, or other deep containers

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