E. coli Gram Staining

E. coli Gram Staining Written and Directed By: Colton Anderson Table of Contents iii Table of Contents Table of iii Introduction .. v Materials & Preparation .. 1 Materials and 3 Materials .. 3 Pre-Lab Preparation .. 3 Gram Stain Lab .. 5 Preparing the organism .. 7 Heat fixing .. 7 Gram Staining .. 8 Viewing Under the Microscope .. 11 Using the Microscope .. 12 Viewing your organism .. 12 Index .. 15 Introduction v Introduction It is important to be aware of the correct way to stain an organism. Without knowing the right way to do it you could potentially harm yourself and your lab partners. In this manual, you will be given direction on how to perform a simple gram stain of the E. coli bacteria. 3 Materials & Preparation In this section, we will gather all of the necessary materials to safely execute the lab procedure. 3 Materials and Preparation Materials 1 Bunsen Burner 1 Inoculating loop 1 Striker (to light the burner) 1 Vial of E.

• A basic light microscope has 4 objective lenses – 4X, 10X, 40X, and 100X. The higher the number, the higher the power of magnification. Once you have focused the microscope, you will cycle between these to get a better image of the organism • The stage controls move the stage side to side, and back and forward.

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