E. coli Gram Staining

← Back to document page

E. coli Gram Staining Written and Directed By: Colton Anderson Table of Contents iii Table of Contents Table of iii Introduction ........................................ ... v Materials & Preparation ............................. 1 Materials and 3 Materials ........................................ ....... 3 Pre-Lab Preparation ................................ 3 Gram Stain Lab ....................................... 5 Preparing the organism ............................ 7 Heat fixing ........................................ .... 7 Gram Staining ....................................... 8 Viewing Under the Microscope .................. 11 Using the Microscope ............................. 12 Viewing your organism ........................... 12 Index ........................................ ............ 15 Introduction v Introduction It is important to be aware of the correct way to stain an organism.

• A basic light microscope has 4 objective lenses – 4X, 10X, 40X, and 100X. The higher the number, the higher the power of magnification. Once you have focused the microscope, you will cycle between these to get a better image of the organism • The stage controls move the stage side to side, and back and forward.

  Light, Coils, Lenses