Transcription of Mini-PROTEAN Tetra Cell - Bio-Rad
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Mini-PROTEAN Tetra CellInstruction ManualCatalog Numbers165-8000 165-8004165-8001 165-8005 165-8002 165-8006165-8003 165-8007 Table of Contents Section 1 General Information 1 Introduction 1 Components 1 Specifications 4 Safety 5 Section 2 Setup and Basic Operation 6 Gel Cassette Preparation 6 Electophoresis Module Assembly and Sample Loading 9 Section 3 Separation Theory and Optimization 15 Introduction 15 SDS-PAGE (Laemmli) Buffer System 16 Native PAGE 17 Section 4 Reagent Preparation and Stock Solutions 19 Volumes Required per Gel 19 SDS-PAGE (Laemmli) Buffer System 19 Discontinuous Native PAGE (Ornstein-Davis) 22 Continuous Native PAGE
®Tetra cell runs both handcast gels and Ready Gel® precast gels interchangeably. The Mini-PROTEAN Tetra system includes a casting stand and glass plates with permanently bonded gel spacers that simplify handcasting and eliminate leaking
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