Transcription of Mouse Embryo Fibroblast (MEF) Feeder
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Mouse Embryo Fibroblast (MEF) Feeder UNIT Cell Preparation The production of Mouse mutants using homologous recombination and blastocyst- mediated transgenesis requires the maintenance of Mouse embryonic stem (ES) cells in an undifferentiated state. Many investigators rely on Feeder layers to prevent ES cell differentiation; Feeder layers prepared from mitotically inactivated primary Mouse em- bryo fibroblasts (MEFs) are used most commonly. This unit describes a simple method to isolate and store MEFs (Basic Protocol 1 and Support Protocol) and two common techniques for mitotic inactivation: -irradiation (Basic Protocol 2) and mitomycin C.
Mouse Embryo Fibroblast (MEF) Feeder UNIT 23.2 Cell Preparation The production of mouse mutants using homologous recombination and blastocyst-mediated transgenesis requires the maintenance of mouse embryonic stem (ES) cells in
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