Transcription of N-Glycan Analysis of Monoclonal Antibodies - IPT Online
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N-Glycan Analysis of Monoclonal Antibodies - IPT Online
Innovations in Pharmaceutical Technology Issue 4222 Laboratory TechnologyKeywordsN-glycan analysisProtein glycosylationMonoclonalantibodies (mAbs)Ultra high performance liquid chromatography (UHPLC)Fluorescence detectionBy Sonja Schneider and Edgar N gele at Agilent TechnologiesA Quaternary LC System, together with fluorescence detection, provides an optimal method for the sensitive and high-resolving Analysis of 2-AB derivatised glycans released from mAbs and other Analysis of Monoclonal AntibodiesProtein glycosylation is one of the most frequently observed post-translational modifications. Mammalian glycoproteins contain three major types of glycans: N-linked, O-linked and glycosylphosphatidylinositol (GPI) lipid anchors, which consist of one or more monosaccharide units.
liquid chromatography (UHPLC) Fluorescence detection By Sonja Schneider and Edgar Nägele at ... can be coupled to Electrospray Ionisation Quadrupole Time-of-Flight Mass Spectrometry (ESI-QToF-MS) for mass and structure ... Peak Retention Calculated Mass Name Structure time mass + AB 1 30.66 1114.5 1234.5 GlcNAcMan 3 GlcNAc 2 2 35.59 1317.6 ...
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