Transcription of Nextera XT DNA Library Prep Kit Reference Guide (15031942)
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Nextera XT DNA Library Prep Kit Reference Guide (15031942)
NexteraXTDNAL ibraryPrepReferenceGuideDocument# document and its contents are proprietary to Illumina, Inc. and its affiliates ("Illumina"), and are intended solely forthe contractual use of its customer in connection with the use of the product(s) described herein and for no otherpurpose. This document and its contents shall not be used or distributed for any other purpose and/or otherwisecommunicated, disclosed, or reproduced in any way whatsoever without the prior written consent of Illumina. Illuminadoes not convey any license under its patent, trademark, copyright, or common-law rights nor similar rights of any thirdparties by this instructions in this document must be strictly and explicitly followed by qualified and properly trained personnel inorder to ensure the proper and safe use of the product(s) described herein.
Dilute the starting material in 10 mM Tris-HCI,pH 7.5–8.5.Incomplete tagmentation causedby contaminants can cause library preparation failure,poor clustering,or low quality sequencing results. PCR Amplicons When starting with PCR amplicons,the PCR amplicon must be > 300 bp. The standardclean up protocol depletes libraries < 500 bp.
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