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NMR of Proteins

NMR of ProteinsNMR of ProteinsDetermining Protein Structures by NMRD etermining Protein Structures by NMR the process of determining a solution structure by NMR is one of measuring many (hundreds/thousands) of short proton- proton distances and angles, and restraining the protein structure with these computationallyH H ..}d1H NMR Spectra of Proteins1H NMR Spectra of Proteins 1D, 1H NMR spectra of even small Proteins are impossible to interpret in any comprehensive manner -normally, only gross statements about secondary structure, tertiary structure, etc.

• based on magnetization transfer via (mostly) one bond J couplings-most of these couplings are large compared to linewidths for moderate sized proteins (~20 kDa)-magnetization transfer is efficient-indirect (1H) detection • provides selective chemical shift correlation-spectral degeneracy minimized Triple Resonance Approach

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