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NMR Spectroscopy

NMR SpectroscopyMRIDrug designStructural biologyMetabonomicsFood qualityApplicationsNMR = Nuclear Magnetic ResonanceBasic PrinciplesSpectroscopic technique, thus relies on the interaction between material and electromagnetic radiationThe nuclei of all atoms possess a nuclear quantum number, I. ( I 0, always multiples of .)Only nuclei with spin number (I) >0 can absorb/emit electromagnetic atomic mass & number: I = 0 (12C, 16O)Even atomic mass & odd number: I = whole integer (14N, 2H, 10B)Odd atomic mass: I = half integer (1H, 13C, 15N, 31P) The spinning nuclei possess angular momentum, P, and charge, and so an associated magnetic moment.

Sensitivity enhancement by polarization transfer Signal sensitivity enhancement by transferring the greater population differences of high- spins onto their spin-coupled low- partners. H1 H2 C2 C1 1H-13C spin pair

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NMR Spectroscopy - Rutgers University

casegroup.rutgers.edu

Applications. NMR Spectroscopy N.M.R. = Nuclear Magnetic Resonance Basic Principles Spectroscopic technique, thus relies on the interaction between material and electromagnetic radiation ... NMR Spectroscopy The Chemical Shift The NMR frequency of a nucleus in a molecule is mainly determined by its gyromagnetic ratio and the strength of the ...

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