Transcription of One-step real-time duplex reverse transcription …
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One-step real-time duplex reverse transcription …
508 BioTechniques Vol. 36, No. 3 (2004)RNA TECHNOLOGIESVol. 36, No. 3 (2004) BioTechniques 509 INTRODUCTIONOf all methods used to quantify mRNA, real-time reverse transcrip-tion PCR (RT-PCR) is considered the most sensitive and accurate one (1). In addition to its wide use in labora-tory research, real-time RT-PCR has the potential for broad application in the diagnoses of functional defects in disease.
508 BioTechniques Vol. 36, No. 3 (2004)
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